ABSTRACT
To observe what happens during those transitions, single-molecule measurements (as opposed to ensemble measurements) are imperative. Indeed, as discussed in Chapter 3, an ensemble observation of any time-dependent molecular property requires the ensemble members to be synchronized. Although it is possible to prepare all the molecules in the same initial state, it is impossible to make them all jump to another state at the same time as we have no control over the random waiting time before the jump happens.1
