ABSTRACT
Keywords .............................................................................................. 134 References ............................................................................................. 134
7.1 ENZYMATIC SYNTHESIS OF 3', 5'-CYCLIC DIGUANYLATE USING FULL-LENGTH DIGUANYLATE CYCLASE OF THERMOTOGA MARITIMA
7.1.1 INTRODUCTION
Using methods of genetic engineering, two strains producing recombinant full-length diguanylate cyclase (DGC) of Thermotoga maritima with native and mutant allosteric sites have been constructed. The obtained strains have several-fold higher DGC-producing level than those described in literature. It has been shown that target enzymes are produced substantially in the form of active inclusion bodies. Seven-fold increase in activity of the T. maritima DGC was caused by mutation in allosteric site. Possibility of applying full-length DGC of T. maritima in the form of inclusion bodies for synthesis of 3′, 5′-cyclic diguanylate has been originally demonstrated.
