Direct Chromosome Preparation from Regenerating Fish Fin Tissue

This protocol for direct chromosome preparation from regenerating fi n tissue is based on the methodology developed by Denton and Howell (1969), Scheel (1972), Kligerman and Bloom (1977), with some modifi cations as described by Völker et al. (2006; 2007). In brief, regenerating fi n tissue is incubated in a diluted Ringer solution containing colchicine, which results in combined and simultaneous mitostatic and hypotonic treatments. The piece of fi n is then fi xed and the tissue minced in aqueous acetic acid to obtain a cell suspension which is dropped onto clean, pre-warmed slides.