ABSTRACT

Nuclear magnetic resonance (NMR) spectroscopy is the study of biomolecules by recording the interaction of an applied radio frequency (RF) field with the nuclei of such molecules, in the presence of a strong external magnetic field. Zeeman first observed the behavior of certain nuclei subjected to a strong magnetic field at the end of the last century, but practical use of the so-called Zeeman effect (refer to Chapter 2) was only made in the 1950s when NMR spectrometers became widely available.