ABSTRACT
Metabolomics is an interdisciplinary study that involves the comprehensive quantitative profiling of metabolites in a target organism using sophisticated analytical technologies. It is a powerful approach that allows researchers to examine variation in total metabolite profiles, and is capable of detecting complex biological changes using statistical multivariate pattern recognition methods (chemometrics; Putri, et al., 2013b). The main analytical technologies in metabolomic studies are either based on nuclear magnetic resonance (NMR) spectroscopy or mass spectrometry (MS). In addition to these two main technologies, Fourier transform-infrared (FTIR) spectroscopy have also been used. Mass spectrometry requires a preseparation of the metabolic components using either chromatography techniques such as gas chromatography (GC) or liquid
chromatography (LC), or other separation techniques such as capillary electrophoresis (CE) coupled to MS (Nicholson, Holmes, and Lindon, 2007).
