ABSTRACT

Phylogenomics is a rapidly growing field of study concerned with using genome-wide data—usually in the form of DNA sequence loci—to infer the evolution of genes, genomes, and the Tree of Life. Accordingly, this discipline connects many areas in biology including molecular and genomic evolution, systems biology, molecular systematics, phylogeography, conservation genetics, DNA barcoding, and others. With the advent of Next Generation Sequencing in addition to advances in computer hardware and software over the past decade, researchers can now generate unparalleled phylogenomic datasets that are helping to illuminate many areas in the life sciences. This book is an introduction to the principles and practices of gathering these data. Phylogenomic Data Acquisition: Principles and Practice is intended for a broad cross-section of biologists and anyone else interested in learning how to obtain phylogenomic data using the latest methods.

chapter 1|11 pages

Introduction

chapter 2|24 pages

Properties of DNA Sequence Loci: Part I

chapter 3|29 pages

Properties of DNA Sequence Loci: Part II

chapter 4|13 pages

DNA Extraction

chapter 5|23 pages

PCR Theory and Practice

chapter 6|26 pages

Sanger Sequencing

chapter 7|63 pages

Illumina Sequencing

chapter 8|25 pages

Developing DNA Sequence Loci

chapter 9|4 pages

Future of Phylogenomic Data Acquisition