ABSTRACT
This chapter discusses the time required for doing deoxyribonucleic acid (DNA) extractions ranges from less than an hour to several days depending on extraction method used and the nature and number of the genetic samples. It provides an overview of the common methods for extracting DNA, describes the advantages and disadvantages of each method, and considers some troubleshooting strategies. Formalin adversely affects DNA in a number of ways including forming cross-linkages between DNA bases and between DNA and proteins; altering the nucleotides; and fragmenting the DNA. The various extraction methods differ from each other in terms of specific procedures and reagents used, they involve the same basic sequence of steps. There are: lysis of tissues/cells and degradation of proteins; degradation of RNA; isolation of DNA from proteins; desalting the DNA; and resuspension of DNA in storage buffer solution. After the extraction process is completed the next task is to check the quality, concentration, and purity of the extracted DNA samples.
