ABSTRACT

INTRODUCTION As vitrication is a cryopreservation technique increasingly applied in clinical practice for reproductive cells and tissue, the main focus of this chapter is to analyze the risks related to this technique and to propose possible solutions. Since there may be concerns regarding the safety of vitrication procedures and cryostorage, due to the contact of cell/tissue/carrier with liquid nitrogen (LN2), these aspects are comprehensively treated in the “Safety of vitrication and cryostorage” section.