ABSTRACT
High-sensitivity isothermal titration calorimetry (ITC; Wiseman et al. 1989) constitutes a particularly useful method for the study of peptide-membrane interactions. A combination of experiments known as uptake and release titrations can be performed and analyzed to assess the thermodynamics of such interactions by means of a surface partition equilibrium (Heerklotz et al. 1999) that is modulated by electrostatics (Keller et al. 2006). is chapter explains the theory of surface partition equilibria and Coulombic membrane eects and demonstrates its implementation in the form of tting equations for the simultaneous analysis of uptake and release experiments, for which the experimental background is also provided and discussed. e theoretical and experimental approach is exemplied using
the CPP penetratin (Keller et al. 2007), and the driving forces underlying its association with lipid membranes are discussed comprehensively.
