ABSTRACT
Inuence of marine microorganisms in the ocean is inexplicable since they pay off more than 95% of the ocean respiration (Del Giorgio and Duarte, 2002). Nevertheless, diversity of the microbes in the marine environment is huge, but the recovery and culture of these organisms in the laboratory is merely 0.1% (Ferguson et al., 1984) for the reason that all the marine microbes are not cultivable under laboratory conditions. However, this lacuna between the microorganisms and the laboratory can be overcome with the advent of advanced nucleic acid technologies; vast phylogenetic diversity is integrated with orthologous gene sequences, and the resulting microbial diversity is at least a minimum of 100 times greater than the previously estimated culture-reliant assessments (Pace, 1997). The 16S rRNA gene-based sequences are used to estimate
the phylogenetic diversity of microorganisms (Giovannoni et al., 1990; Rappe and Giovannoni, 2003), without culturing them.
