ABSTRACT
The basic methodology for manipulating genes and cloning recombinant DNA molecules requires various restricting and modifying enzymes, certain methods for amplifying DNA, and vectors for carrying this recombinant DNA molecule (Hartl et al. 1988; Howe 2007; Nair 2008; Reece 2004). This chapter will introduce some of the basic tools required for cloning and genetic engineering. The following chapters will walk us through the more advanced tools for modifying the sequence of DNA or protein molecules and monitoring the effects of these changes.
