ABSTRACT
GILBERT GREUB, CAROLE KEBBI-BEGHDADI, CLAIRE BERTELLI, FRANÇOIS COLLYN, BEAT M. RIEDERER, CAMILLE YERSIN, ANTONY CROXATTO, AND DIDIER RAOULT
11.1 INTRODUCTION
The recent availability of new generation sequencing technologies [1], [2] has provided unprecedented sequencing capacity, enabling the acquisition of genome-scale sequences at an extraordinary fast rate. These innovative techniques provide amazing opportunities for high-throughput structural and functional genomic researches and have been applied to date to a variety of contexts such as whole-genome sequencing [3] and resequencing [4], targeted resequencing [5], non coding RNA [6] or DNA-binding of modified histones [7], [8]. These high-throughput sequencing methods avoid the need for in vivo cloning and achieve a high accuracy. Even homopolymer problems, i.e. the major drawback of 454 pyrosequencing,
may be overcome by reaching high sequence coverage [1]. These new technologies greatly reduce the work, time and expenses of such projects.
