ABSTRACT
The effect of non-adsorptive immobilization of CAbs on retention of AgCC. Illustrations labelled A and 8 on the left show how a CAb is either adsorbed directly on polystyrene (8) or immobilized via a streptavidin bridge (A) to some adsorbed, biotinylated carrier protein (RGG, KL Y or GEL). Graph C on the right, shows that the AgCC (measured as the percentage of antigen captured) is> 5-fold higher for non-adsorbed CAbs (closed triangles) than for those directly adsorbed. So that the amount of CAb immobilized by each method could be precisely known, initial studies were conducted using 1251-CAb or biotinylated 1251-CAb (6, 29).
