Diagnosis of Chlamydia) Infection in the Pediatric Population I5I I4. DFA, EIA, PCR, LCR, and Other Technologies: What Tests Should be Used for Diagnosis of Chlamydia Infections?

doi:10.1201/9781482269840-22

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Diagnosis of Chlamydia) Infection in the Pediatric Population I5I I4. DFA, EIA, PCR, LCR, and Other Technologies: What Tests Should be Used for Diagnosis of Chlamydia Infections?

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ABSTRACT

For many years, isolation in tissue culture (TC) was considered the test of choice for diagnosis of Chlamydia trachomatis infection. Non-culture tests, such as direct fluorescent antibody (DFA) and enzyme immunoassay (EIA) which detected chlamydia) antigens in clinical specimens, made chlamydia diagnostic tests more widely available. DFA and EIA were less sensitive than TC and had some false positive results which compromised our ability to use these tests in low prevalence settings.