ABSTRACT
Presently, most screening approaches use classical bioassays, which usually test compounds singly in fluorescence or radiotracer competitive enzyme binding assays. High-throughput screening, a variation of this approach, typically carries out multiple assays in parallel with the assistance of automation. In order to reduce the number of assays required to screen a combinatorial library, pools of compounds may be tested for biological activity instead. Then, successively smaller pools of ligands may be screened in an iterative approach until an active compound is isolated. However, this approach is slow and laborintensive, especially when there are several pools in a library that demonstrate activity.
