ABSTRACT
Julian D. Watts, Axel Ducret*, Daniel Figeys, Ming Gu, Yanni Zhang, Paul A. Haynes, Rose Boyle, and Ruedi Aebersold University of Washington, Seattle, Washington
I. INTRODUCTION 282 II. T-CELL RECEPTOR SIGNALING: A COMPLEX,
REGULATED SYSTEM 284 III. AUTOMATED, HIGH-THROUGHPUT IDENTIFICATION OF
PROTEINS BY LC-ESI-MS/MS 290 JV. NOVEL TECHNOLOGIES FOR THE IDENTIFICATION OF
PROTEINS AT THE LOW FEMTOMOLE SENSITIVITY LEVEL 298
v. DETERMINATION OF PHOSPHORYLATION SITES BY LC-MS/MS AND CE-MS/MS 303
VI. SEARCHING FOR AND ANALYSIS OF REGULATORY GLYCOSYLATED PROTEINS 3 I I
VII. A GENOMIC APPROACH TO SEARCHING FOR PROTEIN TYROSINE KINASE SUBSTRATES 315
VIII. CONCLUSIONS 319 REFERENCES 320
282 Watts et al.
