ABSTRACT
Chemiluminescence (CL) is the light emission produced by a chemical reaction in which chemically excited molecules decay to the ground state and emit photons.
Bioluminescence (BL) is a type of CL that occurs naturally in living organisms and is also used in vitro. Measurement of light from a chemical reaction is very useful from an analytical point of view, because in appropriate experimental con ditions the light output intensity is directly related to the analyte concentration, thus allowing precise and accurate quantitative analysis. In addition, the kinetics of light emission is usually a steady-state glow type, which simplifies sample handling and measurement procedures.
