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therefore be on the fourth SCR or on the serine/theonine rich region. By sequencing genomic DNA from Cr(a-) people, Telen and colleagues showed that a mutation in the fourth SCR was responsible for Cr3 [13]. Considering the MAIEA results, the fourth SCR would be a good place to start looking for difference responsible for the WES polymorphism too. Other Cromer system antigens showed some inhibition with one of the BRIC antibodies [12]. MAIEA provided biochemical evidence that Esa is indeed a Cromer system antigen [12]. Esa was thought to be a Cromer related antigen because of the failure of anti-Esa to react with Cromer-null cells and from its behaviour with proteinaese treated cells [14]. These findings were supported by the observation that Esa was carried by a glycosyl phosphatidylinositol linked protein [15]. However, only a small amount of anti-Esa was available and,therefore, immunoblotting experiments could not be done. Strong positive results with BRIC 216 and 110 but a negative result with BRIC 230 suggested that Esa is located on DAF, possibly on the first SCR. Similarly, a negative result with BRIC 230 and Tca suggests that it too is on the first SCR (Table II) [12]. The results of the MAIEA tests for Cromer antigens are summarised in Table II. They agree with those known from DNA studies, Dra on SCR III [15,16,17] and Cr3 on SCR IV [13], and suggest the best places to look for those as yet undetermined. This demonstrates how MAIEA may be used to help narrow the field of study to determine the molecular basis of antigens. VARIATION IN EXPRESSION OF SOME Rh ANTIGENS We had hoped to apply MAIEA to Rh but to date the only antibodies to the D protein are of human origin, so MAIEA cannot yet be used to study the relationship of the D antigen to some of the low incidence antigens which appear to be markers of partial D antigens. The Rh antigen D is, after ABO, the most important antigen clinically because it is highly immunogenic. Until the introduction of Rh immunoprophylaxis, anti-D was the most frequent cause of haemolytic disease of the newborn and neonatal death [1]. Many Rh antigens are good immunogens. Since its initial recognition in the nineteen-forties, the Rh system has become very complex. There are 48 numbered antigens, that is serologically defined determinants, the numbers have reached 50 because two numbers have been declared obsolete [2,3,18,19]. Some antigens are polymorphic and others are of high or low incidence.
DOI link for therefore be on the fourth SCR or on the serine/theonine rich region. By sequencing genomic DNA from Cr(a-) people, Telen and colleagues showed that a mutation in the fourth SCR was responsible for Cr3 [13]. Considering the MAIEA results, the fourth SCR would be a good place to start looking for difference responsible for the WES polymorphism too. Other Cromer system antigens showed some inhibition with one of the BRIC antibodies [12]. MAIEA provided biochemical evidence that Esa is indeed a Cromer system antigen [12]. Esa was thought to be a Cromer related antigen because of the failure of anti-Esa to react with Cromer-null cells and from its behaviour with proteinaese treated cells [14]. These findings were supported by the observation that Esa was carried by a glycosyl phosphatidylinositol linked protein [15]. However, only a small amount of anti-Esa was available and,therefore, immunoblotting experiments could not be done. Strong positive results with BRIC 216 and 110 but a negative result with BRIC 230 suggested that Esa is located on DAF, possibly on the first SCR. Similarly, a negative result with BRIC 230 and Tca suggests that it too is on the first SCR (Table II) [12]. The results of the MAIEA tests for Cromer antigens are summarised in Table II. They agree with those known from DNA studies, Dra on SCR III [15,16,17] and Cr3 on SCR IV [13], and suggest the best places to look for those as yet undetermined. This demonstrates how MAIEA may be used to help narrow the field of study to determine the molecular basis of antigens. VARIATION IN EXPRESSION OF SOME Rh ANTIGENS We had hoped to apply MAIEA to Rh but to date the only antibodies to the D protein are of human origin, so MAIEA cannot yet be used to study the relationship of the D antigen to some of the low incidence antigens which appear to be markers of partial D antigens. The Rh antigen D is, after ABO, the most important antigen clinically because it is highly immunogenic. Until the introduction of Rh immunoprophylaxis, anti-D was the most frequent cause of haemolytic disease of the newborn and neonatal death [1]. Many Rh antigens are good immunogens. Since its initial recognition in the nineteen-forties, the Rh system has become very complex. There are 48 numbered antigens, that is serologically defined determinants, the numbers have reached 50 because two numbers have been declared obsolete [2,3,18,19]. Some antigens are polymorphic and others are of high or low incidence.
therefore be on the fourth SCR or on the serine/theonine rich region. By sequencing genomic DNA from Cr(a-) people, Telen and colleagues showed that a mutation in the fourth SCR was responsible for Cr3 [13]. Considering the MAIEA results, the fourth SCR would be a good place to start looking for difference responsible for the WES polymorphism too. Other Cromer system antigens showed some inhibition with one of the BRIC antibodies [12]. MAIEA provided biochemical evidence that Esa is indeed a Cromer system antigen [12]. Esa was thought to be a Cromer related antigen because of the failure of anti-Esa to react with Cromer-null cells and from its behaviour with proteinaese treated cells [14]. These findings were supported by the observation that Esa was carried by a glycosyl phosphatidylinositol linked protein [15]. However, only a small amount of anti-Esa was available and,therefore, immunoblotting experiments could not be done. Strong positive results with BRIC 216 and 110 but a negative result with BRIC 230 suggested that Esa is located on DAF, possibly on the first SCR. Similarly, a negative result with BRIC 230 and Tca suggests that it too is on the first SCR (Table II) [12]. The results of the MAIEA tests for Cromer antigens are summarised in Table II. They agree with those known from DNA studies, Dra on SCR III [15,16,17] and Cr3 on SCR IV [13], and suggest the best places to look for those as yet undetermined. This demonstrates how MAIEA may be used to help narrow the field of study to determine the molecular basis of antigens. VARIATION IN EXPRESSION OF SOME Rh ANTIGENS We had hoped to apply MAIEA to Rh but to date the only antibodies to the D protein are of human origin, so MAIEA cannot yet be used to study the relationship of the D antigen to some of the low incidence antigens which appear to be markers of partial D antigens. The Rh antigen D is, after ABO, the most important antigen clinically because it is highly immunogenic. Until the introduction of Rh immunoprophylaxis, anti-D was the most frequent cause of haemolytic disease of the newborn and neonatal death [1]. Many Rh antigens are good immunogens. Since its initial recognition in the nineteen-forties, the Rh system has become very complex. There are 48 numbered antigens, that is serologically defined determinants, the numbers have reached 50 because two numbers have been declared obsolete [2,3,18,19]. Some antigens are polymorphic and others are of high or low incidence.
ABSTRACT