ABSTRACT
The use of radioactive tracers in the study of reaction mechanisms has been steadily increasing over the last two decades. Tracer techniques have been used to elucidate the mechanism of complex reactions throughout the field of biotechnology and, in particular, to follow metabolic pathways, both synthetic and natural in both plants and animals. Just as the analytical problems that arose in such work educed the development of GC radioactivity detectors, so have they also evoked the development of LC radioactivity detectors. In LC, however, as the mobile phase is a liquid, radioactivity detection is more difficult and the procedures used for radioactivity detection in GC cannot be used.
