ABSTRACT

All existing systems for performing countercurrent chromatography (CCC) may be classified into two major categories: hydrostatic and hydrodynamic equilibrium systems [1,2]. In the hydrostatic system the mobile phase percolates through the column of the stationary phase on the effect of gravitational or centrifugal force where the mixing of the two phases relies entirely on the flow of the mobile phase. In contrast, the hydrodynamic system produces active phase mixing using a rotating force field induced by the planetary motion of the coil, thus yielding much more efficient solute partitioning.