ABSTRACT
Countercurrent chromatography (CCC) is a separation method based on the partition of solutes between two liquid phases as they interact in a thin tube under a centrifugal force field. One source of interest in this method is that no solid matrix is required to retain the stationary phase. However, optimal settings of the apparatus are difficult to find because many variables or factors are involved. Experimental design methodology (EDM) enables one to solve this problem, and the solution to optimize an 11-factor study is described.
