ABSTRACT
Recombinant DNA technology has advanced rapidly over the past decade. It is now possible to express a large number of important mammalian genes in bacteria or yeast. The overexpression of these genes allows a specific protein to be produced in large quantity-up to 30-40% of the total cellular proteins. Once a protein with commercial value is successfully overexpressed in the cellular system, a cost-effective recovery procedure must be selected. In most cases, the estimated cost of isolating and purifying a recombinant protein can be 80% of the total production cost [1]. Therefore, protein purification plays a pivotal role in the evolution of biotechnology.
