ABSTRACT
Chromatographic separations of solutes are usually based on such parameters as hydrophobicity, molecular charge, and size of the molecules. In chiral separations, where these parameters are common among the racemates, the method requires an additional parameter to obtain enantioselectivity. There are three ways to separate enantiomers: (1) The racemic pair of enantiomers can be derivatized by an optically pure reagent. The two diastereoisomers obtained can be separated by classical liquid chromatography. (2) A chiral molecule can be added to the mobile phase so that diastereoisomers are reversibly formed inside the column and are separated by the classical achiral stationary phase. (3) A chiral selector is bonded to the solid stationary phase, which becomes capable of separating some enantiomeric pairs [1,2].
