ABSTRACT
Coarse Texture .......................................................................................................... 263 14.4.3 Three-Dimensional Reconstruction of Pothos Cells with Glossy Texture .............. 266
14.5 Conclusions ........................................................................................................................... 267 Appendix ........................................................................................................................................ 271
A.14.1 SML Operator ........................................................................................................... 271 A.14.2 MM Operator ............................................................................................................ 271
References ...................................................................................................................................... 272
Recent advances in computerized light microscopy systems have enabled the three-dimensional (3-D) analysis of the structure of objects.1·6 To obtain information on the 3-D architecture of cells and tissues at high magnification, the confocal laser-scanning microscope (CLSM) has been effectively used.4-8 In this system, the 3-D image is typically constructed by stacking numerous twodimensional (2-D) images, which are obtained at consecutive confocal planes. The CLSM has fluorescence imaging capability, and it can provide monochromatic or pseudo-color images. However, this system cannot be used to obtain suitable 3-D full real color RGB images. In addition, using the CLSM for in situ observation of cells and tissues over a wide magnification range under natural growing conditions is difficult. This problem results because, in this situation, the laser is operated at a narrow working distance and must be adjusted, thereby affecting the physiological reactions of the target cells.
