ABSTRACT
The purpose of this final chapter is to provide the analyst with a background of practical examples to aid in the selection of, firstly, the best chromatographic method and, secondly, the best phase system when faced with an hitherto unknown sample for analysis. The literature is rich with LC applications and frequently publications are available for the separation of closely similar mixtures to that of the sample. It is unlikely, however, that the chromatographic conditions for the actual separation required will be available and, even if they are, the conditions reported may well not be optimum. This is more likely to be true for those applications that are described in earlier publications. Nevertheless, conditions that have be successfully employed for related separations may certainly help to identify those conditions necessary for the sample supplied for assay.
