Breadcrumbs Section. Click here to navigate to respective pages.
Chapter
Chapter
Amplified portions of 16S rRNA or specific functional genes from a mixed microbial population might be of similar sizes with a particular set of oligonucleotide primers, but have small differences at the nucleotide level. One way of detecting these differences is to restrict the PCR-amplified product with restriction endonucleases and examine the pattern of restriction fragments (Herrick et al. 1993, Massol-Deya el al. 1997, Smit et 1997). The analytical power of this procedure derives from the
DOI link for Amplified portions of 16S rRNA or specific functional genes from a mixed microbial population might be of similar sizes with a particular set of oligonucleotide primers, but have small differences at the nucleotide level. One way of detecting these differences is to restrict the PCR-amplified product with restriction endonucleases and examine the pattern of restriction fragments (Herrick et al. 1993, Massol-Deya el al. 1997, Smit et 1997). The analytical power of this procedure derives from the
Amplified portions of 16S rRNA or specific functional genes from a mixed microbial population might be of similar sizes with a particular set of oligonucleotide primers, but have small differences at the nucleotide level. One way of detecting these differences is to restrict the PCR-amplified product with restriction endonucleases and examine the pattern of restriction fragments (Herrick et al. 1993, Massol-Deya el al. 1997, Smit et 1997). The analytical power of this procedure derives from the
ABSTRACT
Restriction Analysis of PCR-amplified Products
Denaturing Gradient Gel Electrophoresis (DGGE) and Temperature Gradient Gel Electrophoresis (TGGE)