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or phenotype to enable monitoring in a given environment (Jansson and De Bruijn 1999). These biomarkers therefore fill a surrogate role, supplying an assayable gene product when an assay for a gene product of interest is not available or is very difficult to perform. Two biomarkers which have become extremely attractive for monitoring microbial communities and activity are the genes encoding luciferase enzymes and the green fluorescent protein (GFP). This is mainly due to their ease of use, along with the ability to perform the assays in a nondestructive manner. Such non destructive assays allow for repeated experiments to be performed on the same sample, and so changes of a microbial community can be observed.
DOI link for or phenotype to enable monitoring in a given environment (Jansson and De Bruijn 1999). These biomarkers therefore fill a surrogate role, supplying an assayable gene product when an assay for a gene product of interest is not available or is very difficult to perform. Two biomarkers which have become extremely attractive for monitoring microbial communities and activity are the genes encoding luciferase enzymes and the green fluorescent protein (GFP). This is mainly due to their ease of use, along with the ability to perform the assays in a nondestructive manner. Such non destructive assays allow for repeated experiments to be performed on the same sample, and so changes of a microbial community can be observed.
or phenotype to enable monitoring in a given environment (Jansson and De Bruijn 1999). These biomarkers therefore fill a surrogate role, supplying an assayable gene product when an assay for a gene product of interest is not available or is very difficult to perform. Two biomarkers which have become extremely attractive for monitoring microbial communities and activity are the genes encoding luciferase enzymes and the green fluorescent protein (GFP). This is mainly due to their ease of use, along with the ability to perform the assays in a nondestructive manner. Such non destructive assays allow for repeated experiments to be performed on the same sample, and so changes of a microbial community can be observed.
ABSTRACT