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without the necessity for cultivation of the cells (Rattray et al.1990). The light output is indicative of a metabolically active population of cells, since luciferase enzymes are dependent on cellular activity reserves, or reducing equivalents for bioluminescence. If the cells are growing, the light output is proportional to the number of cells in the sample. However, after long-term incubation in harsh environments, microbial cells often become starved or stressed and the light production from luciferase enzymes declines as a response to the change in cellular energy status (Duncan et al.1994). Therefore, in situbioluminescence is not a reliable indicator of microbial biomass under starvation conditions. The substrate and energy limitations for the luciferase reporter system may be overcome by adding nutrients to the sample in order to activate the microbial population (Meikle et al.1992, Duncan et al.1994). Alternatively, total protein from the environmental sample may be extracted. Then, the energy source can be added directly to the protein extract in vitroand the luciferase enzyme activity can be correlated to the specific luciferase-tagged microbial biomass in the sample (Moller et al.1995).
DOI link for without the necessity for cultivation of the cells (Rattray et al.1990). The light output is indicative of a metabolically active population of cells, since luciferase enzymes are dependent on cellular activity reserves, or reducing equivalents for bioluminescence. If the cells are growing, the light output is proportional to the number of cells in the sample. However, after long-term incubation in harsh environments, microbial cells often become starved or stressed and the light production from luciferase enzymes declines as a response to the change in cellular energy status (Duncan et al.1994). Therefore, in situbioluminescence is not a reliable indicator of microbial biomass under starvation conditions. The substrate and energy limitations for the luciferase reporter system may be overcome by adding nutrients to the sample in order to activate the microbial population (Meikle et al.1992, Duncan et al.1994). Alternatively, total protein from the environmental sample may be extracted. Then, the energy source can be added directly to the protein extract in vitroand the luciferase enzyme activity can be correlated to the specific luciferase-tagged microbial biomass in the sample (Moller et al.1995).
without the necessity for cultivation of the cells (Rattray et al.1990). The light output is indicative of a metabolically active population of cells, since luciferase enzymes are dependent on cellular activity reserves, or reducing equivalents for bioluminescence. If the cells are growing, the light output is proportional to the number of cells in the sample. However, after long-term incubation in harsh environments, microbial cells often become starved or stressed and the light production from luciferase enzymes declines as a response to the change in cellular energy status (Duncan et al.1994). Therefore, in situbioluminescence is not a reliable indicator of microbial biomass under starvation conditions. The substrate and energy limitations for the luciferase reporter system may be overcome by adding nutrients to the sample in order to activate the microbial population (Meikle et al.1992, Duncan et al.1994). Alternatively, total protein from the environmental sample may be extracted. Then, the energy source can be added directly to the protein extract in vitroand the luciferase enzyme activity can be correlated to the specific luciferase-tagged microbial biomass in the sample (Moller et al.1995).
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