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community structure and function, there is a clear trend to combine molecular measures of species composition and the abundance of important microbial groups with measurement of particular processes and environmental parameters. Such studies have the potential to relate community structure to function and activity in complex microbial communities. For example, community structure and function have been analysed through a combination of whole cell in situ hybridization and microsensors. rRNA-based localizations of ammonia- and nitrite-oxidizing bacteria were performed on a nitrifying biofilm following microelectrode measurements for Oj, N20 and N02/N 03' (Schramm et al. 1996, Santegoeds 1998). A good correlation of community structure and function could be demonstrated on a microscopic scale. The distribution of sulfate-reducing and methanogenic bacteria was also determined in a similar manner, with respect to activity (Raskin et al. 1994). Although environmental biotechnology and bioremediation in general could largely benefit from these multidisciplinary analyses of the structure and function of complex microbial communities, the role of classical microbial ecology should not be underestimated. Molecular studies complemented by appropriate culture-based investigations will assist in identifying organisms that are truly representative of those important in nature. It is only by selecting a range of appropriate tools in a complementary fashion that some of the mysteries of microbial ecology can be unlocked and the wealth of novel biodiversity presented by natural microbial communities can be harvested. A.,
DOI link for community structure and function, there is a clear trend to combine molecular measures of species composition and the abundance of important microbial groups with measurement of particular processes and environmental parameters. Such studies have the potential to relate community structure to function and activity in complex microbial communities. For example, community structure and function have been analysed through a combination of whole cell in situ hybridization and microsensors. rRNA-based localizations of ammonia- and nitrite-oxidizing bacteria were performed on a nitrifying biofilm following microelectrode measurements for Oj, N20 and N02/N 03' (Schramm et al. 1996, Santegoeds 1998). A good correlation of community structure and function could be demonstrated on a microscopic scale. The distribution of sulfate-reducing and methanogenic bacteria was also determined in a similar manner, with respect to activity (Raskin et al. 1994). Although environmental biotechnology and bioremediation in general could largely benefit from these multidisciplinary analyses of the structure and function of complex microbial communities, the role of classical microbial ecology should not be underestimated. Molecular studies complemented by appropriate culture-based investigations will assist in identifying organisms that are truly representative of those important in nature. It is only by selecting a range of appropriate tools in a complementary fashion that some of the mysteries of microbial ecology can be unlocked and the wealth of novel biodiversity presented by natural microbial communities can be harvested. A.,
community structure and function, there is a clear trend to combine molecular measures of species composition and the abundance of important microbial groups with measurement of particular processes and environmental parameters. Such studies have the potential to relate community structure to function and activity in complex microbial communities. For example, community structure and function have been analysed through a combination of whole cell in situ hybridization and microsensors. rRNA-based localizations of ammonia- and nitrite-oxidizing bacteria were performed on a nitrifying biofilm following microelectrode measurements for Oj, N20 and N02/N 03' (Schramm et al. 1996, Santegoeds 1998). A good correlation of community structure and function could be demonstrated on a microscopic scale. The distribution of sulfate-reducing and methanogenic bacteria was also determined in a similar manner, with respect to activity (Raskin et al. 1994). Although environmental biotechnology and bioremediation in general could largely benefit from these multidisciplinary analyses of the structure and function of complex microbial communities, the role of classical microbial ecology should not be underestimated. Molecular studies complemented by appropriate culture-based investigations will assist in identifying organisms that are truly representative of those important in nature. It is only by selecting a range of appropriate tools in a complementary fashion that some of the mysteries of microbial ecology can be unlocked and the wealth of novel biodiversity presented by natural microbial communities can be harvested. A.,
ABSTRACT
Anderson, J.B., C. Sternberg, L.K. Poulsen, S.P. Bjorn, M. Givskov, and S. Molin. 1998. New unstable variants of green fluorescent protein for studies of transient gene expression in bacteria. Appl. Environ. Microbiol. 64: 2240-2246.
