ABSTRACT

In addition to profound differences from the rod-like perforatorium in shape and location, the bufonoid conical perforatorium develops very late in spermiogenesis (well after chromatin condensation and nuclear elongation) and in association with the nuclear membrane (Burgos and Fawcett 1956; Rastogi et al. 1988), whereas the endonuclear perforatorium develops very early in spermiogenesis and originates, as we have seen, from a granule at the base of the acrosome vesicle (e.g. Sandoz 1970a). In Odontophrynus cultripes, acid phosphatase was found in the conical perforatorium [labeled acrosome] and glucose-6-phosphatase in the acrosome vesicle [labeled acrosome membrane complex] (Fernandes and Bao 1998).