ABSTRACT
Fig. 6.14 Dorsal skin glands of Rana observed under LM after histochemical trials (A-C. Transverse sections after PAS reaction, contrasted with haemalum-orange, D. Uncontrasted sections after alcian blue 8GX at pH 2,5). A-B. Ordinary and specialized mucous glands of male Rana dalmatina (A) and R. iberica (B) are recognizable on account of their different size and histochemical response. Note that only the ordinary mucous glands are PAS positive. C. Serous and ordinary mucous glands of R. perezi (male) treated with the PAS reaction. Notice the positive response of the ordinary mucous glands (arrows). D. Features of the skin glands in female R. esculenta after the alcian blue test. The ordinary mucous glands react positively (arrows). Labels: ep, epidermis; om, ordinary mucous gland; sg, serous gland; sm, specialized mucous gland. Bars: A-B = 50 pm; C-D = 100 pm. Original.
