ABSTRACT

Creeping bentgrass (Agrostis palustris Huds.) is a popular cool-season, perennial turfgrass widely used on golf courses and tennis greens (Miller 1984). Therefore, the ability to genetically engineer this crop-in order to improve its performance and pest-resistance qualities or to enhance its alternative uses is - of great importance. Successful transformation of creeping bentgrass has recently been reported using either embryogenic callus (Zhong et al., 1993; Hartman et al., 1994), protoplasts (Lee et al.,

'Corresponding author: E-mail: mjcho@nature.berkeley.edu

1996; Asano et al., 1998) or suspension cultures (Xiao and Ha, 1997). However, these approaches involve laborious steps that are difficult to reproduce in terms of initiation and maintenance of cultures.