ABSTRACT
Bastel-Corre et al., (2002) conducted a proteome analysis to study the molecular regulation of SR genes in the model plant Medicago truncatula that was challenged with both nitrogen fixer Sinorhizobium meliloti and AM fungus Glomus mosseae. Time course tests on root protein profiles indicated modifications, either up-or downregulation of several SR genes and a couple of newly induced polypeptides. They utilized matrix assisted laser desorption/ionization-time of flight mass spectrometry, as well as searches using previous databases to conclude that one of the polypeptides relates to M. truncatula hemoglobin. One other polypeptide could be easily attributed as elongation factor TU of S. meliloti. Among the rest, glutathione-stransferase, a fucosidase, a myosin-like protein, a serine hydroxymethyl transferase and cytochrome-c-oxidase were also easily identifiable in mycorrhizal roots. In view of the above results, Bastel-Corre et al., (2002) have opined that proteome analysis could help in detecting molecular changes during the development and functioning of such tripartite symbiosis.
