ABSTRACT
Self-fluorescence of studied pure compounds added on the subject glass was small, but the emission increased 5-10 times after their interactions with the microspores. After the cell treatment with muscarine, dtubocurarine, atropine, diOHATN, mesaton, yohimbine, labetalol, prazosin and tavegyl microspores emitted, mainly, in blue (max. 450-470 nm) whereas BODIPY-DA, BODIPY-5HT and Kur-14 - in green (max. 518-520 nm). The green or blue rings of the studied agonists or antagonists were seen under the luminescent microscope on the cellular surface where they were bound with proposed receptors. Among studied agents, BODIPYderivatives of neurotransmitters, muscarine, 6-7-diOHATH, dtubocurarine, yohimbine and Kur-14 may be recommended as possible fluorescent probes for the study of cellular location of cholinoreceptors and receptors of biogenic amines. Besides, blue-fluorescent alkaloids capsaicin from Capsicum annuum L., prototype of vanilloid receptor (see Chapter 3), and arecoline from Areca catechu L., agonist of acetylcholine, have been, perhaps, perspective as fluorescent dyes. Anthraquinone hypericin, bounded on the cell surface (see colour Fig. 26 in Appendix 2), inhibits protein kinases (Haugland, 2000) and has an affinity to glutamate Nmethyl-D-aspartate receptor (Kubin et al., 2005). This compound related to surface receptors also may be fluorescent dye that needs special investigations in a future.
