ABSTRACT
Subcutaneous (SQ) injections can be administered easily to mice. Restrain by the scruff and insert the needle between the tented folds of skin. Pull back on the plunger of the syringe to verify that a vacuum is created, then inject the solution. In general, no greater than 1 ml should be injected per subcutaneous injection site (25-g adult mouse). Use several sites over the back of the animal if larger volumes must be administered. You can inject up to 10 ml/kg (maximum is 40 ml/kg) of liquid. Performing one-handed injections is necessary for this technique.
