ABSTRACT
Although affinity chromatography can employ a variety of ligands, most of these tend to be of biological origin. The use of a biological ligand within a column is sometimes known as bioaffinity chromatography or biospecific adsorption. This was the first type of affinity chromatography developed, as used by Starkenstein in 1910 when he purified αamylase using insoluble starch [1]. The specificity of most biological ligands makes bioaffinity chromatography an ideal technique for the purification of numerous compounds. This method has also become important as a tool in the analysis and characterization of biological samples.
