ABSTRACT
The performance of electrophoresis in narrow bore capillaries obviated most of the functions of gels in electrophoresis, e.g., elimination of convection (through rapid dissipation of Joule heat) and reduced diffusion (through short analysis time). However, another important feature of gels is their capability to actively participate in the separation process by providing a sieving medium that differentially affects the Inigration velocity of sample components according to molecular size. Macromolecules such as nucleic acids and SDSprotein complexes exhibit no significant mobility differences during free zone electrophoresis, and require the presence of an interactive, sieving separation matrix.
