ABSTRACT

AnalyticalUltracentrifugation.Analyticalultracentrifugationcanbeusedtoyieldequilibriumconstantvaluesforavarietyofantigen-antibodysystems(28).Themethodreliesonthe factthatantigens,antibodies,andAg-Abcomplexeshavedifferentsedimentationvelocitiesso that,uponseparationinagravitationfield,theywillseparateandtheamountofeachcanbe quantitatedoptically(e.g.,withschlierenoptics)asthesurfaceareaundereachpeak.