ABSTRACT

In this chapter we will describe some of the current technologies used on gene isolation and analysis in plants. To date, a diverse array of tech­ niques for genetically transformed plants has been conceived and some of them can be applied to most of the important crop species. As stated, an

essential step toward the improvement of plants by genetic engineering is the incorporation of the appropriate traits. Therefore, the possibility to manipulate important agricultural traits in many crop species will depend in large extent on the knowledge of the genetics and biochemistry of ba­ sic processes in plant biology. Genes encoding most of these traits are un­ known, so are the regulatory mechanisms governing the expression of them; that is why a great deal of effort is now centered on gene discovery.