ABSTRACT
The hybridization of nucleic acids is a fundamental tool of molecular biology to detect a target DNA sequence. It is very frequently used to screen cDNA or genomic libraries, to study the organization of specific regions of the genome (by Southern blot), or to analyse the accumulation of transcripts in cells. The success of these techniques depends on the possibility of obtaining labelled DNA probes using radioactive or chemically modified nucleotides. Markers can be used to obtain probes labelled either uniformly (internal labelling) or at their ends (end labelling).
