ABSTRACT
Many genome sequencing projects result in the characterization of genes of unknown function or genes identified by simple homology of sequence. One way to demonstrate the biological function of these genes is to complement a mutation of the function studied in yeast. Genetic transformation can be used to integrate the target gene in the mutant yeast that has lost a known function, then the phenotype of the transformed yeast is determined in order to discover whether the introduced gene is capable of recovering the lost function or, in other words, complementing the mutation.
