ABSTRACT
In conventional techniques of genetic transformation (see Profiles 33 to 37), transformed cells are selected on a selective medium inhibiting the growth of non-transformed cells. The cells, tissues, or plants thus obtained are then analysed for the expression of the gene or genes introduced. The obtaining of transgenic organisms is undoubtedly a fundamental tool for the study of gene expression, but the procedures to obtain these organisms are often time-consuming, even difficult for some species. Transient expression can be used for rapid analysis of the expression of a gene without selection of transformed cells. Such studies can then be complemented by the analysis of these promoters in transgenic organisms (see Profile 30).
