ABSTRACT
High-Performance Liquid Chromatography (HPLC) is a modern technique widely used for both analytical and preparative separation of compounds contained in more or less complex mixtures of different origin. Analytes separation is obtained, after the injection of the sample mixture, in a selected column containing an appropriate stationary phase and subjected to the action of a liquid (mobile phase) pumped at a relative high pressure (up to 400-500 bar) with a constant ow-rate. The stationary phase can be present in the column in different ways, e.g., either as packed particles of varied nature or polymers or monoliths that can be bonded or adsorbed on the wall. Compounds are separated according to their physical-chemical properties, also considering the characteristics of the mobile and stationary phases.
