ABSTRACT

The determination of antioxidant activity (capacity or potential) of diverse biological samples is generally based on the inhibition of a particular reaction in the presence of antioxidants. The most commonly used methods are those involving chromogenic compounds of a radical nature: the presence of antioxidant leads to the disappearance of these radical chromogens. They are either photometric or fluorimetric and can comprise kinetic or end-point measurements. Recently, there has been increasing interest in the adaptation of these methods for on-line determinations using liquid chromatography. In this article, we present the adaptation to high-performance liquid chromatography (HPLC) of our methods for the determination of the antioxidant activity in a range of samples. Advantages and disadvantages of these methods are discussed.