ABSTRACT
The high-speed countercurrent chromatography (HSCCC) centrifuge uses a multilayer coil as a separation column which produces high-efficiency separation with good retention of the stationary phase with a variety of two-phase solvent systems.[1,2] However, the method often fails to retain a satisfactory amount of the stationary phase for highly viscous, low interfacial solvent systems such as polymer phase systems, which are useful for the separation of macromolecules and particulates. A new column design called “spiral disk assembly,” described below, provides a universal application of solvent systems, including the polymer phase systems for HSCCC.
