ABSTRACT
High speed countercurrent chromatography (HSCCC) produces highly efficient chromatographic separations of solutes without the use of solid supports.[1-3] Thus the method eliminates all complications caused by the solid support, such as adsorptive loss and deactivation of samples, tailing of solute peaks, contamination, etc. As with other CCC schemes, HSCCC utilizes two immiscible solvent phases, one as a stationary phase and the other as a mobile phase, and the separation is highly dependent on the partition coefficient values of the solutes, i.e., the ratio of the solute concentration between the mobile and stationary phases. Therefore the successful separation necessitates a careful search for the suitable two-phase solvent system that provides an ideal range of the partition coefficient values for the applied sample.
