ABSTRACT
Since Jorgenson and Lukacs[1] separated peptides in 1981 using free zone electrophoresis in glass capillaries, capillary electrophoresis (CE) has become a highly efficient technique for the separation of biopolymers such as proteins, peptides, carbohydrates, and DNA. Free solution CE, or capillary zone electrophoresis (CZE), is an electrophoresis in free homogeneous solution.[2,3] Charged solutes are simply separated on the basis of the solute charge-to-mass ratio, applied electric field, and the pH and ionic strength of the background electrolyte (BGE).
