ABSTRACT
The two-dimensional (2-D) thin-layer chromatography (TLC) technique is one of the more versatile methods of TLC development. The first application of the 2-D chromatographic method to paper chromatography was reported in 1944 by Consden, Gordon, and Martin.[1] Since that time, this method has been mostly used for the separation of a large number of compounds that cannot be separated in a one-dimensional (1-D) TLC experiment. In 2-D TLC, separation is on one surface spread along the entire area of the plate. The resolving power of the 2-D TLC method has great application, especially in the areas of biochemistry, biology, natural products, pharmaceuticals, and environmental analysis.
