ABSTRACT

First published in 1998. This book is targeted at the beginner who has little or no knowledge of the principles or practice of hybridization. This text concentrates on solution and filter hybridization with a final chapter on current developments which includes DNA chips and advances in probe design.

chapter 1|10 pages

Introduction to nucleic acid hybridization

chapter 6|8 pages

Basic types of filter hybridization

chapter 7|12 pages

Hybridization strategy: long probes

chapter 9|8 pages

Choice of probe

chapter 11|22 pages

Basic techniques: labeling of probes

chapter 13|19 pages

Basic techniques: detection of hybrids

chapter 14|4 pages

Troubleshooting

chapter 15|34 pages

Specific applications of techniques

chapter 16|6 pages

Current trends