ABSTRACT
SEOG-JIN SEO ], IN-KYU PARK l, MI-KYOUNG YOO [, MAYUMI SHIRAKAWA 2, TOSHIHIRO AKAIKE3 and CHONG-SU CHO K* 1 School of Agricultural Biotechnology, Seoul National University, Seoul 151-742, South Korea
2 Food, Food Additives and Chemicals Division, Dainippon Pharmaceutical Co., Suita, Osaka 564-0053, Japan Department of Biomolecular Engineering, Tokyo Institute of Technology, Yokohama 226-8501, Japan
Received 23 September 2003; accepted 27 March 2004
Abstract-The possibility of employing naturally derived xyloglucan (XG) having galactose moieties in the side chain for the development of synthetic extracellular matrix in tissue engineering was studied. Hepatocyte adhesion to the XG-coated polystyrene (PS) dish was 73.9% after 30 min incubation, whereas that to the PS dish as a negative control was 59.1%. The hepatocyte adhesion to the XG-coated surface was dependent on the presence of Ca2+ ions, whereas that to the XG-coated surface could not be induced by Mg2+ ions alone, indicating specific interaction between galactose moieties of XG and asialoglycoprotein receptors of hepatocytes. From the results of fluorescence, confocal laser micrographs and flow cytometry, it was suggested that XG was internalized by hepatocytes through a receptor-mediated mechanism. The DNA synthesis of hepatocytes attached to the XG-coated surface was decreased with an increase of the coating concentration of XG and in the presence of epidermal growth factor (EGF). The spreading shapes of the hepatocytes attached to the surface in the presence of EGF at low concentration of XG (1 /xg/ml) were enhanced. The hepatocytes attached to the surface at a high concentration of XG (200 /xg/ml) showed round shapes with spheroids after 16 h in the presence of EGF.
